Evaluation of five commercial real-time PCR assays for detection of Mycoplasma pneumoniae in respiratory tract specimens.
نویسندگان
چکیده
The performances of five commercial TaqMan real-time PCR assays for the detection of Mycoplasma pneumoniae in respiratory tract specimens were evaluated in comparison with an in-house real-time PCR. All kits allowed prompt and specific results, validated by the use of an internal control. The Nanogen kit showed the best clinical sensitivity.
منابع مشابه
Evaluation of five real - time PCR assays for detection of Mycoplasma pneumoniae 7 8 Running title : Real - time PCR for detection of
25 Four commercial real-time PCR assays to detect M. pneumoniae were tested and the results 26 compared with an in-house approach. Despite differences of crossing threshold values up to 4, 27 assays were able to detect at least 20 cfu/5 μl (52 fg DNA/5 μl) of sample with best clinical 28 sensitivity of Diagenode kit. 29
متن کاملEvaluation of five real-time PCR assays for detection of Mycoplasma pneumoniae.
Four commercial real-time PCR assays to detect Mycoplasma pneumoniae were tested, and the results were compared with the results for an in-house approach. Despite differences of crossing threshold values of up to 4, assays were able to detect at least 20 CFU/5 μl (52 fg DNA/5 μl) of sample with the Diagenode kit showing the best clinical sensitivity.
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 47 7 شماره
صفحات -
تاریخ انتشار 2009